The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.
Identifieur interne : 000646 ( Main/Exploration ); précédent : 000645; suivant : 000647The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.
Auteurs : Long Lin [République populaire de Chine] ; Wenwu Ye [République populaire de Chine] ; Jiawei Wu [République populaire de Chine] ; Mingrun Xuan [République populaire de Chine] ; Yufei Li [République populaire de Chine] ; Jian Gao [République populaire de Chine] ; Yonglin Wang [République populaire de Chine] ; Yan Wang [République populaire de Chine] ; Suomeng Dong [République populaire de Chine] ; Yuanchao Wang [République populaire de Chine]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2018.
Abstract
Transcriptional regulation is critical for plant pathogen development and virulence. MADS-box transcription factors belong to a highly conserved transcriptional regulator family in eukaryotic organisms that are involved in various important biological processes. Only one predicted MADS-box gene, PsMAD1, was identified in Phytophthora sojae, which was highly expressed during the sporangia and infection stages. To investigate its function, we generated PsMAD1 knockout mutants using the CRISPR/Cas9 system. Compared with the wild-type strain, the mutants showed no changes in vegetative growth, oospore production, or no differences in sensitivity to various abiotic stresses. Although sporangia production was normal, no zoospore release was detected in PsMAD1 mutants. Microscopy analyses revealed failure of cleavage of the cytoplasm into uninucleate zoospores in the mutants. In addition, the mutants showed reduced virulence in soybean. RNA-seq data indicated that PsMAD1 may regulate many zoospore development and infection associated genes. Thus, PsMAD1 may be a major regulator of P. sojae involved in zoosporogenesis and pathogenesis.
DOI: 10.3389/fmicb.2018.02259
PubMed: 30319576
PubMed Central: PMC6165875
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of <i>Phytophthora sojae</i>.</title><author><name sortKey="Lin, Long" sort="Lin, Long" uniqKey="Lin L" first="Long" last="Lin">Long Lin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Jiawei" sort="Wu, Jiawei" uniqKey="Wu J" first="Jiawei" last="Wu">Jiawei Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xuan, Mingrun" sort="Xuan, Mingrun" uniqKey="Xuan M" first="Mingrun" last="Xuan">Mingrun Xuan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yufei" sort="Li, Yufei" uniqKey="Li Y" first="Yufei" last="Li">Yufei Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Jian" sort="Gao, Jian" uniqKey="Gao J" first="Jian" last="Gao">Jian Gao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yan" sort="Wang, Yan" uniqKey="Wang Y" first="Yan" last="Wang">Yan Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30319576</idno><idno type="pmid">30319576</idno><idno type="doi">10.3389/fmicb.2018.02259</idno><idno type="pmc">PMC6165875</idno><idno type="wicri:Area/Main/Corpus">000649</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000649</idno><idno type="wicri:Area/Main/Curation">000649</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000649</idno><idno type="wicri:Area/Main/Exploration">000649</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of <i>Phytophthora sojae</i>.</title><author><name sortKey="Lin, Long" sort="Lin, Long" uniqKey="Lin L" first="Long" last="Lin">Long Lin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Jiawei" sort="Wu, Jiawei" uniqKey="Wu J" first="Jiawei" last="Wu">Jiawei Wu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xuan, Mingrun" sort="Xuan, Mingrun" uniqKey="Xuan M" first="Mingrun" last="Xuan">Mingrun Xuan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yufei" sort="Li, Yufei" uniqKey="Li Y" first="Yufei" last="Li">Yufei Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Jian" sort="Gao, Jian" uniqKey="Gao J" first="Jian" last="Gao">Jian Gao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yan" sort="Wang, Yan" uniqKey="Wang Y" first="Yan" last="Wang">Yan Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transcriptional regulation is critical for plant pathogen development and virulence. MADS-box transcription factors belong to a highly conserved transcriptional regulator family in eukaryotic organisms that are involved in various important biological processes. Only one predicted MADS-box gene, <i>PsMAD1</i>, was identified in <i>Phytophthora sojae</i>, which was highly expressed during the sporangia and infection stages. To investigate its function, we generated <i>PsMAD1</i> knockout mutants using the CRISPR/Cas9 system. Compared with the wild-type strain, the mutants showed no changes in vegetative growth, oospore production, or no differences in sensitivity to various abiotic stresses. Although sporangia production was normal, no zoospore release was detected in <i>PsMAD1</i> mutants. Microscopy analyses revealed failure of cleavage of the cytoplasm into uninucleate zoospores in the mutants. In addition, the mutants showed reduced virulence in soybean. RNA-seq data indicated that PsMAD1 may regulate many zoospore development and infection associated genes. Thus, <i>PsMAD1</i> may be a major regulator of <i>P. sojae</i> involved in zoosporogenesis and pathogenesis.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30319576</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of <i>Phytophthora sojae</i>.</ArticleTitle><Pagination><MedlinePgn>2259</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2018.02259</ELocationID><Abstract><AbstractText>Transcriptional regulation is critical for plant pathogen development and virulence. MADS-box transcription factors belong to a highly conserved transcriptional regulator family in eukaryotic organisms that are involved in various important biological processes. Only one predicted MADS-box gene, <i>PsMAD1</i>, was identified in <i>Phytophthora sojae</i>, which was highly expressed during the sporangia and infection stages. To investigate its function, we generated <i>PsMAD1</i> knockout mutants using the CRISPR/Cas9 system. Compared with the wild-type strain, the mutants showed no changes in vegetative growth, oospore production, or no differences in sensitivity to various abiotic stresses. Although sporangia production was normal, no zoospore release was detected in <i>PsMAD1</i> mutants. Microscopy analyses revealed failure of cleavage of the cytoplasm into uninucleate zoospores in the mutants. In addition, the mutants showed reduced virulence in soybean. RNA-seq data indicated that PsMAD1 may regulate many zoospore development and infection associated genes. Thus, <i>PsMAD1</i> may be a major regulator of <i>P. sojae</i> involved in zoosporogenesis and pathogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Long</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Wenwu</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Jiawei</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xuan</LastName><ForeName>Mingrun</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yufei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yonglin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Suomeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuanchao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CRISPR/Cas9</Keyword><Keyword MajorTopicYN="N">MADS-box transcription factor</Keyword><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">gene knockout</Keyword><Keyword MajorTopicYN="N">pathogenicity</Keyword><Keyword MajorTopicYN="N">sporangia cleavage</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>09</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30319576</ArticleId><ArticleId IdType="doi">10.3389/fmicb.2018.02259</ArticleId><ArticleId IdType="pmc">PMC6165875</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Science. 1990 Nov 16;250(4983):931-6</Citation><ArticleIdList><ArticleId IdType="pubmed">17746916</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2011 Apr;80(1):33-53</Citation><ArticleIdList><ArticleId IdType="pubmed">21276092</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 1;313(5791):1261-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16946064</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2008 May;7(5):791-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18344407</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2003 Oct 16;316:1-21</Citation><ArticleIdList><ArticleId IdType="pubmed">14563547</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1990 Jul 5;346(6279):35-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1973265</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2016 Jan;17(1):127-39</Citation><ArticleIdList><ArticleId IdType="pubmed">26507366</ArticleId></ArticleIdList></Reference><Reference><Citation>Mech Dev. 2002 Sep;117(1-2):201-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12204259</ArticleId></ArticleIdList></Reference><Reference><Citation>Semin Cancer Biol. 1990 Feb;1(1):47-58</Citation><ArticleIdList><ArticleId IdType="pubmed">2133110</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2006 Jun;4(6):435-44</Citation><ArticleIdList><ArticleId IdType="pubmed">16710324</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 2018 Feb 14;46:7-13</Citation><ArticleIdList><ArticleId IdType="pubmed">29454192</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2017 Apr;214(1):361-375</Citation><ArticleIdList><ArticleId IdType="pubmed">28134441</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2013 Dec;30(12):2725-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24132122</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Microbiol. 2003 Oct;11(10):462-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14557029</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Proteomics. 2014 Aug;13(8):2101-13</Citation><ArticleIdList><ArticleId IdType="pubmed">24872595</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1988 Dec 23;55(6):989-1003</Citation><ArticleIdList><ArticleId IdType="pubmed">3203386</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 1989 Jul;3(7):921-35</Citation><ArticleIdList><ArticleId IdType="pubmed">2673922</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2007 Jan;8(1):1-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20507474</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2014 Sep 11;15:785</Citation><ArticleIdList><ArticleId IdType="pubmed">25214042</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2011 Dec;24(12):1530-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21848399</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2000 May 9;97(10):5328-33</Citation><ArticleIdList><ArticleId IdType="pubmed">10805792</ArticleId></ArticleIdList></Reference><Reference><Citation>J Cell Sci. 2010 Dec 15;123(Pt 24):4374-81</Citation><ArticleIdList><ArticleId IdType="pubmed">21098641</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2013 Jul 17;14:483</Citation><ArticleIdList><ArticleId IdType="pubmed">23865555</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1990 Mar;9(3):605-13</Citation><ArticleIdList><ArticleId IdType="pubmed">1968830</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Gen Genet. 1987 Apr;207(1):142-8</Citation><ArticleIdList><ArticleId IdType="pubmed">3298999</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2008 Dec;7(12):2133-40</Citation><ArticleIdList><ArticleId IdType="pubmed">18931042</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2002 Apr 15;363(Pt 2):377-86</Citation><ArticleIdList><ArticleId IdType="pubmed">11931668</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2017 Feb 23;18(1):198</Citation><ArticleIdList><ArticleId IdType="pubmed">28228125</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 May 3;102(18):6496-501</Citation><ArticleIdList><ArticleId IdType="pubmed">15845764</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2013 Nov;159(Pt 11):2259-68</Citation><ArticleIdList><ArticleId IdType="pubmed">23985144</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013 Mar;9(3):e1003182</Citation><ArticleIdList><ArticleId IdType="pubmed">23516354</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2004 Oct;17(10):1051-62</Citation><ArticleIdList><ArticleId IdType="pubmed">15497398</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2002 Oct;1(5):687-95</Citation><ArticleIdList><ArticleId IdType="pubmed">12455688</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1994 Nov 11;22(22):4673-80</Citation><ArticleIdList><ArticleId IdType="pubmed">7984417</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2005 Jan;3(1):47-58</Citation><ArticleIdList><ArticleId IdType="pubmed">15608699</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Dev. 1991 Dec;5(12A):2327-41</Citation><ArticleIdList><ArticleId IdType="pubmed">1748287</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci China Life Sci. 2011 Dec;54(12):1143-50</Citation><ArticleIdList><ArticleId IdType="pubmed">22227907</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2003 Oct;50(2):487-94</Citation><ArticleIdList><ArticleId IdType="pubmed">14617173</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Long" sort="Lin, Long" uniqKey="Lin L" first="Long" last="Lin">Long Lin</name></noRegion><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name><name sortKey="Gao, Jian" sort="Gao, Jian" uniqKey="Gao J" first="Jian" last="Gao">Jian Gao</name><name sortKey="Gao, Jian" sort="Gao, Jian" uniqKey="Gao J" first="Jian" last="Gao">Jian Gao</name><name sortKey="Li, Yufei" sort="Li, Yufei" uniqKey="Li Y" first="Yufei" last="Li">Yufei Li</name><name sortKey="Li, Yufei" sort="Li, Yufei" uniqKey="Li Y" first="Yufei" last="Li">Yufei Li</name><name sortKey="Lin, Long" sort="Lin, Long" uniqKey="Lin L" first="Long" last="Lin">Long Lin</name><name sortKey="Wang, Yan" sort="Wang, Yan" uniqKey="Wang Y" first="Yan" last="Wang">Yan Wang</name><name sortKey="Wang, Yan" sort="Wang, Yan" uniqKey="Wang Y" first="Yan" last="Wang">Yan Wang</name><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><name sortKey="Wu, Jiawei" sort="Wu, Jiawei" uniqKey="Wu J" first="Jiawei" last="Wu">Jiawei Wu</name><name sortKey="Wu, Jiawei" sort="Wu, Jiawei" uniqKey="Wu J" first="Jiawei" last="Wu">Jiawei Wu</name><name sortKey="Xuan, Mingrun" sort="Xuan, Mingrun" uniqKey="Xuan M" first="Mingrun" last="Xuan">Mingrun Xuan</name><name sortKey="Xuan, Mingrun" sort="Xuan, Mingrun" uniqKey="Xuan M" first="Mingrun" last="Xuan">Mingrun Xuan</name><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000646 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000646 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30319576
|texte= The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30319576" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |